Abstract:

Objective To study the pharmacological mechanism of honeysuckle and forsythia for treatment of influenza based on network pharmacology and molecular docking analysis,and to provide evidence for its clinical application.Methods The main active chemical components of "honeysuckle-forsythia" were obtained from TCMSP database，and the relevant targets were queried.The influenza targets were retrieved through OMIM，Gencards，TTD，GKB and Drug Bank databases;the interacting proteins were analyzed on the String platform.The PPI network was constructed，and the main protein function modules were found in the network.Run Bioconductor data package in R language to analyze "drug-component-target" and enrich relevant biological processes and pathways.The results are visualized and then Cytoscape 3.8.0 software was used to build the drug pair component-disease target network.Finally，the analysis and docking were carried out，and the software AutoDock was used.Results The core active ingredients of honeysuckle-forsythia for treatment of influenza were apigenin，quercetin and ursolic acid.The core targets included IL-6，IL-1β，RELA，MAPK14，JUN，etc.Conclusion "Honeysuckle-forsythia" affects the release of immune cells by acting on influenza biological pathways such as AGE-RAGE signal pathway and IL-17 signal pathway.This paper expounds the acting mechanism of this drug pair in regulating and treating influenza and provides basis and ideas for clinical application

Key words:

Influenza, Honeysuckle, Forsythia, Network pharmacology, Molecular docking