Abstract: Objective：To explore the establishment of successful nephritis hematuria rat model at low prices. Methods：Based on intragastric administration and regular tail vein injection of bovine serum albumin (BSA),hematuria in nephritis was produced by a tail intravenous injection of enterotoxin B (SEB) or damp-heat factors in rats respectively.Then,biological methods and light microscope were employed for comparative observations on the two ways of inducing nephritis hematuria. Results：Both of the two methods succeeded in duplicating mesangial proliferative glomerulonephritis (MsPGN) with obvious hematuria and typical pathological changes in rats.An enhanced damp-heat factor could raise rat weight,aggravating edema and hematuria,as well as elevating the levels of blood urea nitrogen. Conclusion：The damp-heat group shows less effectiveness in the extent of hematuria and proteinuria than the control group with SEB injection；however，it’s more accord with the “Damp-heat Syndromes” in clinical TCM,thus proved to be an economical animal model of nephritis hematuria with typical pathological changes.

Key words: Nephritis;Hematuria;Model;Damp-heat syndrome;MsPGN；Animal, experiment