ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2014, Vol. 6 ›› Issue (2): 122-125.doi: 10.3969/j.issn.1674-3865.2014.02.010

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肌肽预处理对缺氧缺血后大鼠认知功能的影响

张香敏，张展，程秀永，徐发林，贾天明，栾斌，贾莉婷   

  1. 450052 郑州，郑州大学第三附属医院儿科(张香敏，徐发林，贾天明，栾斌)，检验科(张展，贾莉婷)；450000 郑州，郑州大学第一附属医院新生儿科(程秀永)
  • 收稿日期:2013-11-08 出版日期:2014-04-25 上线日期:2014-04-25
  • 通讯作者: 张展
  • 作者简介:张香敏(1966-)，女，医学博士，主治医师。研究方向：新生儿脑损伤的诊断与治疗。

The effect of carnosine pretreatment on cognition function of HI rat

ZHANG Xiang min，ZHANG Zhan，CHENG Xiu yong，et al.   

  1. Department of Pediatrics，the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052，China
  • Received:2013-11-08 Published:2014-04-25 Online:2014-04-25

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摘要： 目的:探讨肌肽预处理对缺氧缺血后大鼠认知功能的影响。方法:SD大鼠36只，出生后7 d结扎并切断左侧颈总动脉，吸入8%氧氮混合气2 h即制成缺氧缺血(HI)模型，随机将SD大鼠分为3组(每组12只)：(1)肌肽预处理组：在HI诱导前30 min腹腔注射肌肽1次250 mg/kg；(2)HI组：在相同条件下用等容积的生理盐水代替；(3)假手术组：不接受任何治疗和处理。大鼠在HI后21~26 d通过Morris水迷宫实验的定位航行试验及空间探索实验，评价肌肽对大鼠游泳时间、距离及在目标象限游泳时间的百分比的影响。结果:肌肽预处理降低了HI大鼠的游泳时间和游泳距离，增加了HI大鼠在目标象限游泳时间的百分比。结论:肌肽预处理改善了HI大鼠的认知功能，从而有效地发挥了神经保护作用。

关键词: 缺氧缺血性脑病, 窒息, 肌肽, 认知功能, 大鼠, 动物, 实验

Abstract: Objective:To explore the effects of carnosine pretreatment on cognitive function in hypoxic ischemia (HI) rats.Methods:A total of 36 SD rats，7 days after birth,were ligated and cut off the left common carotid artery;they inhaled 8% oxygen and nitrogen mixture for 2 h to induce HI，and then these SD rats were randomly divided into 3 groups (n=12)：(1) carnosine pretreatment group：given intraperitoneal injection of carnosine 250 mg/kg at a time 30 min before the HI induction；(2) the HI groups：under the same condition , the same volume of physiological saline was used instead；(3) control group：no treatment or management was given.Some 21~26 days after HI,  by Morris water maze experiment——navigation trial and space probe trial，the effect of carnosine on swimming time，distance，and percentage of time spent in the target quadrant was evaluated.Results:Carnosine pretreatment reduced swimming time，shortened swimming distance，and increased percentage of time spent in the target quadrant of HI rats.Conclusion:Carnosine pretreatment improves the cognitive function of the HI rats，hence effectively plays its role in the nerve protection.

Key words: hypoxic-ischaemic cerebropathy, asphyxia, carnosine, diagnosis application, cognitive function, rat, animal, test