ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2014, Vol. 6 ›› Issue (2): 126-129.doi: 10.3969/j.issn.1674-3865.2014.02.011

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孤独症模型动物认知能力与胃肠功能发育状况的观察

张兴，李丽娜，林孟柯，宁艳哲，凌雅睿，田小珑，王茂晋，张忠   

  1. 100029 北京，北京中医药大学第一临床医学院2009级中医学七年制(临床方向)(张兴，林孟柯，宁艳哲，凌雅睿，田小珑)；100029 北京，北京中医药大学基础医学院科研中心(李丽娜)，人体形态学解剖教研室(张忠)；100029 北京，北京中医药大学管理学院2009级公共事业管理(王茂晋)
  • 收稿日期:2013-10-29 出版日期:2014-04-25 上线日期:2014-04-25
  • 通讯作者: 张兴
  • 作者简介:张兴(1989-)，男，北京中医药大学2009级七年制硕士研究生在读。研究方向：经方治疗疑难杂病的分子机制。
  • 基金资助:
    北京中医药大学大学生科研课题(201210026067)

Observation on the development of cognitive ability and gastrointestinal function in autism animal models

ZHANG Xing,LI Li na,LIN Meng ke,et al.   

  1. The First Clinical Medical College of Beijing University of Chinese Medicine,Beijing 100029,China.
  • Received:2013-10-29 Published:2014-04-25 Online:2014-04-25

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摘要： 目的:探讨孤独症模型动物认知能力的变化与胃肠功能的发育状况。方法:采用妊娠12.5 d孕鼠尾静脉注射丙戊酸钠(注射用水稀释为250 g/L，600 mg/kg)，所产11只仔鼠为模型组幼鼠；对照组孕鼠注射同等剂量的生理盐水，所产14只仔鼠为对照组幼鼠。观察两组小鼠在生长发育过程中的变化，并记录体质量、游泳、胃肠感觉功能、胃肠运动功能、避暗反应、旷场实验的相关数据，分析其认知发育的变化及胃肠功能的发育状况。结果:与对照组相比，模型组小鼠出现生长发育迟缓、游泳协调能力差、胃肠痛阈降低，胃肠运动功能亢进；认知发育方面，模型组小鼠旷场试验运动距离较短，避暗反应具有更长的潜伏期，且错误次数更少，差异有统计学意义(P＜0.05)。结论:孤独症模型幼鼠生长发育过程更加迟缓，胃肠感觉运动出现障碍。另外，对未知事物的探索较少，对危险刺激因素具有更强的记忆能力。

关键词: 孤独症, 发育, 胃肠功能, 大鼠, 动物, 实验

Abstract: Objective:To investigate the changes of cognitive ability and the development status of gastrointestinal function in animal models with autism.Methods:The rate at 12.5 d pregnancy was injected sodium valproate through the tail vein during pregnancy (injection diluted with 250 g/L，600 mg/kg).The 11 rats produced were regarded as model group rats；another pregnant rat was injected the same dose of normal saline，who produced 14 rats as control group rats.Observe two group of mice on the changes in the process of growth and development，record the data about the weight，swimming，gastrointestinal sensory function，gastrointestinal motility，darkness reaction，and the open field test，analyze the changes of cognitive ability and the development status of gastrointestinal function.Results:Compared with the control group，model group mice showed growth retardation， poorer coordination in swimming，reduced gastrointestinal pain，and hyper function of gastrointestinal motility；in the aspects of cognitive development，the model mice showed shorter movement distance in the open field test,longer incubation period and fewer mistakes,and the difference was of statistical significance.Conclusion:Autism models have more sluggish growth and development process，and experience gastrointestinal problems in both sensory function and motility.In addition，the exploration of the unknown area is less，and they have stronger memory ability as to the dangerous factors.

Key words: autism model, cognitive development, gastrointestinal function, rat, animal, experiment