Abstract: Objective：To compare the serum Mycoplasma pneumoniae antibody ELISA test with indirect blood coagulation test method, and to explore the high cost-effective method in laboratory diagnosis of Mycoplasma pneumoniae infection.Methods：Totally 352 cases of bronchial pneumonia in children hospitalized in Department of Paediatrics of Children's Hospital of Hunan Province from December 2013 to January 2015 were included, and respiratory secretions and serum were made ELISA and indirect hemagglutination test, and serum was isolated and cultured. The separate culture was regarded as the gold standard for the detection. ELISA and indirect hemagglutination test were compared on diagnosis effect.Results：The sensitivity of indirect hemagglutination test method was 97.5%(159/163), significantly higher than that of ELISA(86.5%,141/163)(P<0.05); The specificity of ELISA method for the detection was 91.5%(173/189), significantly higher than indirect hemagglutination method(70.9%,134/189), the difference being statistically significant(P<0.05). The diagnostic efficiency of ELISA detection method was 89.2%, the Kappa index being 0.782; the diagnostic efficiency of indirect hemagglutination method was 83.2%, and the Kappa index was 0.670. The diagnostic efficiency of the two methods was similar, and the Kappa index was in a high degree of consistency.Conclusion：ELISA test and indirect blood coagulation test method have their own advantage, so the laboratory can choose the appropriate method according to the clinical diagnosis.

Key words: Mycoplasma pneumonia, Separate culture, Serology, Immune, Children