Abstract: Objective：To study the effects of two-step sequential therapy on signal pathway of NF-κB of airway inflammation in asthmatic mice.Methods：Forty healthy female Kunming mice were divided into four groups randomly(n=10,in each group):normal control group,asthma group,budesonide group and two-step sequential therapy group.The asthmatic models were established by ovalbumin,and budesonide and two-step sequential therapy were applied to them.Then the pathologic changes in airway tissues and bronchoalveolar lavage fluid(BALF) in cytology were observed.The level of nuclear factor-κB(NF-κB) and GATA-3 in airway tissues was detected by immunohistochemical method.The level of interleukin-4（IL-4） in BALF was detected by enzyme-linked immunosorbent assay(ELISA).Results：The treatment groups had less inflammatory cell infiltration than asthmatic group(P<0.01),and more than normal control group(P<0.01).There was no significant difference between the budesonide group and two-step sequential therapy group(P>0.05).The level of NF-κB,GATA-3 and IL-4 decreased in the budesonide group and two-step sequential therapy group compared with the asthmatic group(P<0.01),but increased compared with the normal control group(P<0.01).There was no significant difference between budesonide group and twostep sequential therapy group(P>0.05).Conclusions：The two-step sequential therapy may inhibit the activity of the signal transduction pathway of NF-κB,which can reduce the expression of GATA-3 and class cytokines of Th2,hence plays an important role in treating asthmatic mice．

Key words: Asthmatic；Ginseng-schisandrae decoction;Minor green-blue dragon decoction;Nuclear factor-κB;GATA-3;Interleukin4；Mice；Animal, experiment