ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2021, Vol. 13 ›› Issue (1): 1-.doi: 10.3969/j.issn.1674-3865.2021.01.001

• 实验论著 •    下一篇

miRNAs小鼠哮喘模型中的作用初探

张冬姣，徐浩   

  1. 110024 沈阳，沈阳医学院附属中心医院儿科
  • 收稿日期:2020-09-08 出版日期:2021-02-25 上线日期:2021-02-25
  • 通讯作者: 徐浩，E-mail:xuhao6668@126.com

Role of miRNAs in the model of asthma in mice

ZHANG Dongjiao，XU Hao   

  1. Pediatric Department，Central Hospital Affiliated to Shenyang Medical Colloge，Shenyang 110024，China
  • Received:2020-09-08 Published:2021-02-25 Online:2021-02-25
  • Contact: XU Hao，E-mail:xuhao6668@126.com

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摘要： 目的：探讨小鼠哮喘模型中miRNAs的差异表达情况。方法：将24只BALB/c小鼠随机分为正常对照组、卵清蛋白处理组、地塞米松治疗组各8只，使用血细胞分析仪检测每组肺泡灌洗液中嗜酸性粒细胞(EOS)、嗜中性粒细胞(NEU)、巨噬细胞(MAC)和淋巴细胞(LYM)的表达情况，通过ELISA的方法检测每组肺泡灌洗液中白细胞介素-4（IL-4）、IL-6和IL-13的表达情况。miRNAs芯片分析不同组别肺组织中差异性表达miRNAs的情况，统计分析miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的相关性。结果：卵清蛋白处理组中EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达显著高于正常对照组，地塞米松治疗组与卵清蛋白处理组相比各种因子表达下调。卵清蛋白处理组多个miRNAs表达发生变化，miR-455在卵清蛋白处理组上调显著。miR455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达呈正相关。结论：miR-455在卵清蛋白诱导的小鼠哮喘模型中显著上调。

关键词: 哮喘, 微小RNA, 炎症因子, 细胞因子

Abstract: Objective：To investigate the differential expression of miRNAs in asthma model of mice.Methods：BALB/c mice were randomly divided into normal control group， asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group)， 8 mice in each group. Eosinophil(EOS)， neutrophil(NEU)， macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4，IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 was statistically analyzed.Results：The expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13  in the asthma group was significantly higher than that in the control group, and the expression of various factors was down regulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed，and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13.Conclusion：miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

Key words: Asthma, MicroRNA, Inflammatory factor, Cytokine