ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2010, Vol. 2 ›› Issue (1): 8-12.doi: 10.3969/j.issn.1674-3865.2010.01.004

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早期行为疗法降低仔鼠脑损伤致脑性瘫痪的实验研究

庞伟，李晓捷，张士岭
  

  1. 154003 黑龙江 佳木斯，佳木斯大学附属第三医院小儿神经科(庞伟),小儿脑瘫科(李晓捷);佳木斯大学儿童神经康复实验室(庞伟,李晓捷,张士岭)
  • 收稿日期:2010-01-10 出版日期:2010-02-25 上线日期:2010-02-25
  • 通讯作者: 李晓捷
  • 作者简介:庞伟（1976-），男，主治医师。研究方向：小儿脑损伤发病机制及早期防治研究。

Experimental study of the early behavior therapy to reduce cerebral palsy of the young rats due to brain damage

PANG Wei，LI Xiao jie，ZHANG Shi ling.   

  1. Department of Pediatric Neurology，the Third Affliated Hospital of Jiamusi University，Jiamusi 154003,China
  • Received:2010-01-10 Published:2010-02-25 Online:2010-02-25

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摘要： 目的：研究早期行为疗法对降低仔鼠脑损伤所致脑性瘫痪（简称脑瘫）的影响。方法：取孕18 d大鼠21只连续2 d腹腔注射脂多糖，剂量为450 μg/kg，6只孕鼠腹腔注射同剂量的生理盐水。随机选取脂多糖组仔鼠分为干预组（n=20）和非干预组（n=30），另设生理盐水组（n=30）；生后2 d起，给予干预组仔鼠早期丰富环境刺激等行为疗法，另两组常规饲养；于生后25 d对3组仔鼠进行随意运动、姿势、肌张力、不自主运动、情感行为能力、倾斜板试验等检测，记录各项评分并进行统计学分析；取生后1 d的非干预组、生理盐水组仔鼠各10只、生后25 d的3组仔鼠各10只进行脑组织髓鞘碱性蛋白(myelin basic protein，MBP)、S100蛋白阳性细胞检测。结果：免疫组织化学显示：生后1 d的非干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较生理盐水组仔鼠均明显减少、减弱(P<0.01)；生后25 d的干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较非干预组仔鼠均增多(P<0.05),生理盐水组仔鼠脑组织各部位(内囊、海马、胼胝体)的MBP阳性染色较干预组、非干预组仔鼠明显增多(P<0.01)；生后1，25 d的非干预组仔鼠脑组织各部位(内囊、海马、胼胝体)的S100蛋白阳性染色与生理盐水组仔鼠相比较，均明显增加、增强（P<0.01），干预组介于两者之间，差异有统计学意义（P<0.01）。神经行为学检测显示：干预组20只仔鼠中有4只鉴定为脑瘫鼠，非干预组有8只脑瘫鼠，而生理盐水组无脑瘫鼠。结论：MBP、S100蛋白可以作为脑损伤早期诊断及判断预后的指标；早期干预可降低宫内感染所致新生鼠脑瘫的发生。

关键词: 脑性瘫痪；脑损伤；行为疗法；大鼠；动物, 实验

Abstract: Objective：To study the effect of early behavior therapy to reduce cerebral palsy caused by brain damage in rats.Methods：Totally 27 pregnant rats were consecutively injected with LPS(450 μg/kg，n=21) or saline(n=6) on gestation 18 d and 19 d.We selected randomly saline neonatal rat group B(n=30) and LPS neonatal rat group A1(n=20)，A2(n=30).The 2 d rats(A1) were given early behavior therapy，such as enriched environment and the rats(A2 and B) were routinely fed.The neurobehavior of the rats was observed in A1，A2 and B groups on 25 d.The MBP and S100 were detected in brain sections on 1 d and 25 d.Results：MBP expression was obviously decreased in A2(1 d),compared with B of 1 d rats(P<0.01);MBP was increased in A1 of 25 d,compared with A2 of 25 d(P<0.05)；it was increased in B,contrasted with A1 and A2(P<0.01).Compared with B,there was significant increase in A2 of 1 d with regard to S100(P<0.01).The result of S100 between A2 and B of 25 d was the same with 1 d rats,but the S100 of A1 was the middle of them（P<0.01）．The result of neurobehavioral observation indicated that there were 4 cerebral palsy rats in A1,8 cerebral palsy rats in A2 and no cerebral palsy rat in B.Conclusion：MBP and S100 can become the sign of early diagnosis and the therapeutic effect in brain injury.Early interference can reduce cerebral palsy.

Key words: Cerebral palsy;Brain damage;Behavior therapy;Rat；Animal, experiment